ProjectMutation accumulation in asexual Daphnia pulex
Mutation is the most fundamental process of evolution yet little is known about the mutation properties and rates in most organisms. In this study, I am performing whole-genome sequencing on 44 independent Daphnia lineages that have been accumulating spontaneous mutations for 80-100 generations. I am sequencing two different Daphnia species and including some lineages that have reduced fitness, in addition to some individuals that have been maintained in a competitive environment where selection is acting. This project will inform on the rates, patterns, biases, and fitness consequences of mutations in this organism.
Publications1- Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Flynn, Jullien M., Emily A. Brown, Frédéric J. J. Chain, Hugh J. MacIsaac, Melania E. Cristescu
2015 Ecology and Evolution